The American Civil Liberties Union and the ACLU of Alaska have filed a brief seeking to allow transgender individuals to correct the gender marker on their driver's licenses without undergoing major surgery. Currently, Alaska DMV policy requires that individuals wishing to change the sex marker on their driver's license submit proof of a surgical "sex change". The state's surgery requirement places an undue burden on transgender individuals and presents a gross violation of an individual's right to privacy.

As part of our "Don't Filter Me" campaign, the American Civil Liberties Union and the ACLU of Eastern Missouri filed a federal lawsuit against a school district in Camdenton, Missouri, whose Internet filtering software blocks access to web content geared toward the lesbian, gay, bisexual and transgender (LGBT) communities. The ACLU is joined in the lawsuit by LGBT organizations whose websites are blocked by the filter: Parents, Family and Friends of Lesbian and Gays (PFLAG), the Matthew Shepard Foundation, Campus Pride, and DignityUSA, a Catholic LGBT organization.

Jennifer Keeton, a student at Augusta State University (ASU), sought a court order requiring ASU to reinstate her in a graduate-level counseling program even though she insisted on a right – rooted in her religious beliefs – to counsel lesbian, gay and bisexual clients that being gay is immoral. ASU's counseling program requires its graduate students to adhere to the American Counseling Association's Code of Ethics, which prohibits counselors from discriminating based on sexual orientation, among other characteristics, and requires them to avoid imposing their values on their clients. The American Civil Liberties Union and the ACLU of Georgia filed a friend-of-the-court brief supporting ASU's right to require its students to comply with these professional standards when counseling clients.

When graduating senior Ceara Sturgis chose to wear a tuxedo for her senior yearbook photo, rather than the drape typically reserved for girls, her school, Wesson Attendance Center, responded by excluding her entirely from the senior portrait section of the yearbook. The ACLU seeks damages for Ceara and hopes to prevent gender discrimination in the future.